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Objective To explore the measurement of(1,3)-β-D glucan in plasma for the diagnosis of pulmonary fungal infections in pulmonary tuberculosis patients. Methods 40 pulmonary tuberculosis patients with pulmonary fungal infections in Guangzhou chest hospital from January 2015 to December 2015 were enrolled as a test group,among which 35 were confirmed and 5 were suspected pulmonary fungal infections. 52 pulmonary tuber-culosis patients without fungal infections were selected as a control group.(1,3)-β-D glucan content(G test)in this 92 patients plasma were detected. The results of G tests were compared with those from etiological diagnosis to assess the performance of G test. Results 13 strains of candida albicans,13 strains of aspergillus,2 strains of candida tropicalis,2 strains of candida glabrata and 6 strains of other yeast were obtained from patients of test group,but no fungal identified from those of control group. The median of G test in test group and in control group was 126.1 and 29.56 pg/mL,respectively,the level in test group was significantly higher than that in control group (P<0.001). 35 cases were identified as positive and 5 were negative in test group by G test ,while 41 cases were identified as negative and 11 were positive in control group. The sensitivity,specificity,positive predictive value, negative predictive value ,concordance and Youden index of G test were 87.5%,78.85%,76.09%,89.13%, 82.6%and 0.663,respectively. Conclusions Candida albicans and aspergillus are more common pathogens than the other fungi isolated from pulmonary tuberculosis patients with pulmonary fungal infection. G test ,used in pul-monary tuberculosis with pulmonary fungal infections diagnosis,is reliable and fast,and has a higher sensitivity, specificity and accuracy.
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BACKGROUND: Hydroxyapatite/chitosan (HA/CS) complex may act as a drug carrier for drug release, but little is reported about the release amount and antibacterial effect of minocycline-HA/CS (Mino-HA/CS) complex. OBJECTIVE: To investigate the in vitro release and antibacterial property of Mino-HA/CS complex. METHODS: HA/CS and Mino-HA/CS were prepared using co-precipitation method. The surface and cross-section features of the complexes were observed under scanning electron microscopy. The porosities were measured according to Archimedes Principle. The release of minocycline hydrochloride was measured by high performance liquid chromatography with the simulated saliva as drug release media. In vitro antibacterial effect on Porphyromonas gingivalis and Staphylococcus aureus were measured by bacteria-inhibiting ring method. Biological toxicities were evaluated via cel counting kit-8cel proliferation assay. RESULTS AND CONCLUSION: The porosity of Mino-HA/CS was larger than that of HA/CS, with the average porosity of 53.99%. Single-day release amount of Mino-HA/CS could maintain at the level of 0.5-1 μg per day for a long-term. Bacteriostatic rings of Porphyromonas gingivalis and Staphylococcus aureus stil existed clearly after 7 days. Cel proliferation assays showed that Mino-HA/CS extract had the significant effect on promoting cel proliferation. These findings indicate that the Mino-HA/CS sustains the release of minocycline at a relatively stable level within a longer period, shows good inhibitory effect on Porphyromonas gingivalis and Staphylococcus aureus and promotes the proliferation of periodontal ligament cel s.
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PURPOSE: To explore whether there is matching relation between the length and the tilting angle of terminal implants in the All-on-Four protocol by studying the effects of different implant configurations on stress distributions of implant, bone, and framework. MATERIALS AND METHODS: Four implants were employed to support a full-arch fixed prosthesis and five three-dimensional finite element models were established with CT images, based on the length (S and L) and distal tilt angle (0degrees, 30degrees and 45degrees) of terminal implants for an edentulous mandible, which named: Tilt0-S, Tilt30-S, Tilt30-L, Tilt45-S and Tilt45-L. An oblique 240 N was loaded at second molar. The von Mises Stresses were analyzed. The implants were consecutively named #1 to #4 from the loading point. RESULTS: 1) Tilt0-S had the greatest stress on the implants, with the other groups exhibiting variable reductions; the four implants of Tilt45-L demonstrated the greatest reduction in stress. 2) Tilt0-S had the greatest stress at bone around #1 implant neck, and Tilt45-L exhibited the least stress, which was a 36.3% reduction compared to Tilt0-S. 3) The greatest stress in the framework was found on the cantilevers distal to #1 implant. Tilt45-S exhibited the least stress. CONCLUSION: Matching different length and tilting angle of the terminal implants led to variable stress reductions on implants, bone and the superstructure. By optimizing implant configuration, the reduction of stress on implants and surrounding bone could be maximized. Under the present condition, Tilt45-L was the preferred configuration. Further clinical testings are required.
Subject(s)
Finite Element Analysis , Mandible , Molar , Neck , Prostheses and ImplantsABSTRACT
Bone morphogenetic proteins (BMPs) are members of the transforming growth factor-β(TGF-β) superfamily and play critical roles in skeletal development, bone formation and stem cell differentiation.BMP9 is one of the most osteogenic BMPs, promoting osteogenesis differentiation of periodontal ligament stem cells ( PDLSCs) both in vitro and in vivo.Recently, signal transduc-tion studies have revealed that BMP-Receptor-Smads and BMP-Receptor-Mitogen activated protein kinase ( MAPK) play vital roles in BMP9 which promote PDLSCs osteogenesis differentiation.Several studies revealed that transcription factors closely associated with os-teogenesis differentiation are found in the downstream of the Smads and MAPK pathways.This review aims to summarize our current knowledge of BMP9-mediated osteogenesis by presenting recently completed works which may help us to further elucidate these path-ways.
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Being a new non-ceramic hydroxyapatite artificial bone substitute with biological activity , calcium phosphate cement is widely applied in clinical therapy due to its biocompatibility , osteoconductivity , degradability and possibility of being drug carriers . In addition, it also has a good performance in root canal filling , apexification, plerosis of pulp floor perforation and alveolar defects . However , it is necessary to resolve the issues , such as selecting appropriate hardening time and how to enhance compressive strength . This article presents a review of the research progress on the biological properties and clinical application of calcium phosphate cement in stomatology .
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Objective：To investigate the changes of calcitonin gene-related peptide immunoreactive (CGRP-IR)nerve fibers in rat dental pulps during acute inflammation. Methods： Rat acute pulpitis model was established by silk thread ligation and the change of CGRP-IR nerve fibers was observed with immunohistochemical method.Results： In radical pulp,the CGRP-IR nerve fibers became denser and more heavily stained；in the coronal pulp,the number of CGRP-IR nerve fibers decreased,but the background staining was heavier. Conclusion： During acute inflammation,the amount of CGRP increases in dental pulps, and is released into the surronding tissue in a large scale in the coronal region.
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Objective:To investigate the distribution of calcitonin gene-related peptide immunoreactive(CGRP-IR) nerve fibers in periodontal tissue of rats, providing morphological data of CGRP in periodontitis. Methods:Five adult SD rats were sacrificed by transheart perfution fixation with 4% paraformaldehyde. The mandibles were removed and decalcified. Frozen sections were made and CGRP-IR nerve fibers in periodontal tissue were observed with immunohistochemical technique. Results: CGRP-IR nerve fibers were observed in gingiva, alveolar bone .periodontal membrane; many heavily stained CGRP-IR nerve fibers were found beneath junctional epithelium, epithelium of gingival sulcus and epithelium of gingival col; some CGRP-IR nerve fibers even penetrated into junctional epithelium and epithelium of gingival col. Conclusion; CGRP-IR nerve fibers extensively disdtribute in the periodontal tissue with the densest in the initial area of periodontitis, suggesting that CGRP may play a role in the genesis and development of periodontitis.
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Objective: To provide theoretical basis and experimental evidence for diagnosis and therapy of dental pulp indirect injuries by gunshot wounds. Methods: The levels of IL 8 in different stages were studied using immunohistochemical method to evaluate its functions in inflammation process of dental pulps. Results: IL 8 expression was detected by immunohistochemical assay in postwound conditions. The results showed that positive staining of IL 8 was observed predominantly on infiltrating inflammatory cells, vascular endothelial cells and some fibroblasts, which was consistent with the recruitment and activation of inflammatory cells, and with the severity duration of pulpal tissue injury and response. Conclusion: These results indicate that IL 8 is involved in regulating physiologic responses of dental pulp injuries in maxillofacial trauma by a projectile. [
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Objective: To investigate the effect of endocrine disturbance on occurrence of periodontitis. Methods: Sixty four SD male rats were divided randomly into four groups (control, simple ligation, simple hormone, hormone and ligation). Experimental periodontitis was initiated by ligation on day 15 of cortisone acetate injection. Mesial alveolar bone height and periodontal histologic changes were observed. Results: Compared with the other three groups, the height of mesial alveolar bone decreased obviously ( P
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Objective:To investigate the relationship of TNF-A-863 and CGRP979 gene polymorphisms with the susceptibility to severe chronic periodontitis in Chinese.Methods:Buccal swabs were collected from 100 adult patients with severe chronic periodontitis and 118 healthy adult controls.DNA was extracted from each subjects of the two groups.PCR-LDR technique was used to identify the genotypes of TNF-A-863 and CGRP979.The difference in the genotypes between the two groups was analyzed by statistics software.Results:The genotype of TNF-A-863 was mainly TNF-A-863 A/C in patients with severe chronic periodontitis and TNF-A-863 C/C in healthy controls.There were significant differences in TNF-A-863 distribution between the two groups(P
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Objective:To compare the root filling results of 2 thermoplasticized gutta-percha filling techniques(Ultrafil 3D and ObturaⅡ) with cold lateral condensation of gutta-percha.Methods: Sixty freshly extracted maxillary mandibular single-rooted teeth were instrumented and randomly divided into 3 groups: Ultrafil 3D,ObturaⅡ and cold lateral condensation groups.The root canal was routinely prepared and the obturation time was recorded.Three days later,10 teeth of each group were randomly stained and the micro effusion length was subsequently measured.The micro-chink between filling materials and the wall of root canal were measured with scanning electron microscope in the other 10 teeth of each group.The data were analyzed using unpaired Student's t-test.Results: The longest obturation time was in cold lateral condensation group,then ObturaⅡ and Ultrafil 3D group in order,with significant difference found between the cold lateral condensation group and the latter 2 groups((P